Cellular Imaging (Lake Nona)

Overview

The Cellular Imaging Lake Nona Facility broadly supports research programs by providing access to sophisticated microscopes for digital imaging, as well as training, assistance and guidance, helping scientists from any institution on a first come-first served basis.
The facility offers expertise, training and assistance in advanced biological microscopic imaging techniques and use of complex image processing software, use of well-maintained, aligned, and calibrated microscopic equipment, and troubleshooting of equipment and experimental problems.
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Services

Widefield Microscopy

    • Brightfield and Darkfield
    • Phase and Nomarski Differential Interference Contrast (DIC)
    • Epifluorescence
    • Evanescent Wave (TIRF)
    • Live Cell and Fixed Specimen

Confocal Microscopy

    • Point Scanning
    • Resonant Scanning
    • Spectral
    • VAAS Detection (Virtual Adaptable Aperture System)
    • Live Cell and Fixed Specimen

Electron Microscopy

    • Transmission
    • Digital Capture
    • Fixed Specimen
    • Image Analysis
    • Traning and Consulation

Equipment and Resources

  • Olympus InvertedIX71: Brightfield, Phase and Widefield Fluorescence microscope with cooled, color and monochrome CCD camera (SPOT Pursuit 14 bit 1360x1024, Diagnostic Instruments). Runs on SPOT Advanced or Image Pro.
  • Nikon Inverted TIRF : Brightfield, DIC and Widefield Fluorescence and Evanescent Wave (TIRF) fully motorized microscope with PFS (Perfect Focus System). Equipped for FRET and Ca2+ imaging, with a cooled monochrome EMCCD camera (QuantEM 16bit 512x512, Photometrics) and a cooled monochrome CCD camera (CoolSNAP HQ2 14bit 1392x1040, Photometrics). Lamdba DG-5 Xenon lamp, Mercury Intensilight, Ar-ion laser emitting at 488 and 514 nm and diode laser emitting at 561 nm. Runs on Nikon Elements Software.
  • NikonA1R VAAS : Laser Point- and Resonant-Scanning Confocal Microscope equipped with single photon Ar-ion laser emitting at 457, 477, 488 and 514 nm; Diode lasers, emitting at 402, 561 nm, and 638 nm. 32-channel spectral detection and deconvolution, FRAP, FRET, photoactivation, high-speed resonant scanning or high-resolution point scanning, ultra-sensitive VAAS detector and Perfect Focus (PFS). Runs on Nikon Elements
  • SoftwareFEI Morgagni Electron Microscope With CCD Camera

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