Stem Cells (La Jolla)

Services

Equipment and Resources for the Maintenance, Manipulation and Image Acquisition

• XVivo Hypoxia Culturing System
A self-contained incubation system that allows one to culture, manipulate and visualize their cultures all under a user specified hypoxic or normoxic condition.

• Two Leica MZ6 Dissecting Stereomicroscopes (Bright field/Dark field)
These microscopes are housed in separate EnviroCo laminar flow hoods for the routine manipulation of stem cell cultures.

• Two Leica M165 FC Fluorescent Dissecting Stereomicroscopes
These microscopes will be available for use for the routine maintenance of stem cell lines that contain fluorescent reporters. One will be housed in a 4 foot EnviroCo laminar flow hood and one will be housed in the XVivo Hypoxia System (See above).

• Leica DMI 4000B Inverted Fluorescent Microscope
This microscope will be mainly available for image acquisition and will have available both the Metavue and the standard Leica image acquisition software.

• Olympus IX71 Inverted Fluorescent Microscope
This microscope will be mainly available for image acquisition and will have available both the Metavue and the standard Olympus image acquisition software.

• MVE 1830 Eterne Cryostorage System
This -190 degree vapor phase cell storage system has the capacity to store ~80,000 2 ml vials. This unit will have a back-up N2 source in addition to a 24-hour remote alarm.

• Custom Biogenic Solutions 2100 Controlled Rate Freezing System: This unit will allow us to “fine-tune” the rate of freezing for stem cells and their derivatives.
  
• Roche Lightcycler 480, RT-PCR
This newly released Lightcycler from Roche allows one to perform reactions in either a 96- or 384- well format. The main feature, in addition to the high quality nature of Roche products, is the ease to switch from the 96- to 384- well format without having to recalibrate the instrument.

• Two 6 foot and four 4 foot Tissue Culture Hoods
For the general maintenance and manipulation of cultures

• Three 4 foot Envirco Laminar Flow Dissection Hoods
For the manual manipulation of cultures.

• Fourteen Sanyo CO2 Tissue Culture Incubators
Which have a UV decontamination system that sterilizes the air as it passes through the chamber.

• Two Olympus CKX-41 Tissue Culture Scopes
For the general visualizations basic image acquisition of cultures

• Three Sanyo upright -80⁰ Freezers
• Three upright 4⁰ Kenmore Refrigerators
• Three upright -20⁰ Kenmore manual defrost freezers
• Beckman Coulter Spectrophotometer
• Two Eppendorf Mastercycler EP Gradient PCR
• Eppendorf 5810R Refrigerated Swing Bucket Centrifuge with capability for multi-well plates
• Eppendorf 5702 Swing Bucket Centrifuge
• Eppendorf 5424 24-spot Fixed Rotor Centrifuge
• Various Shakers, Water Bathes & Other Equipment

Back to Top

Products

Back to Top

Useful Links

• NIH: What are Stem Cells?
• NIH: Frequently Asked Questions Surrounding Stem Cells and Their Potential
• NIH Stem Cell Registry (Publicly Funded)
• ATCC Available Cell Lines
• National Stem Cell Bank
• WiCell
• GlobalStem
• ISSCR Stem Cell Briefings
• Stem Cell Training Courses
• University of California, San Francisco
• University of California, San Diego
• Stanford University
• University of California, Los Angeles
• Harvard Stem Cell Institute

Back to Top

Protocols

Back to Top

Regulations

Sanford-Burnham Institute Policies

CIRM & Proposition 71

CIRM Regulations for Using Awarded Funds for Stem Cell Research

Back to Top