Protein Production and Analysis (La Jolla)

Overview

We provide a variety of protein production services including cloning, expression optimization, development of protein purification protocols, and protein preparation. E. coli, insect (SF9, baculovirus) and mammalian (293) systems are available for protein expression. Our analytical services are focused on biophysical characterization of structural and functional properties of proteins in solution, under native, non-denaturing conditions. We can perform quality control of protein samples (folding, stability, aggregation, etc.); characterize protein conformation and shape in solution; and measure protein binding to proteins, peptides, small molecules and other ligands (including determination of equilibrium (Kd) and kinetic rate (kon, koff) constants, stoichiometry, binding enthalpy and entropy).
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Services

Protein Production

    • Cloning
    • Protein expression trials
    • Protein preparation/purification
    • Optimization of protein purification
    • Cell pellets/conditioned media production

Protein Analysis

    • Absorbance spectroscopy
    • Analytical ultracentrifugation (AUC)
    • Atomic force microscopy
    • Differential scanning calorimetry (DSC)
    • Fast kinetics, stopped flow
    • Fluorescence spectroscopy
    • FPLC, analytical runs
    • Isothermal titration calorimetry (ITC)

Equipment and Resources

  • Bacterial, insect/baculovirus and mammal expression systems
  • Absorbance diode-array spectrophotometer, Agilent 8453 UV/Vis
  • Analytical ultracentrifuge,BeckmanCoulter ProteomeLabâ„¢ XL-I
  • Atomic Force microscope, Agilent 5500 AFM/SPM
  • Cell homogenizer, Avestin EmulsiFlex-C3
  • Differential scanning calorimeter, CSC Nano II DSC
  • Fluorescence spectrometer/fluorometer, BioLogic MOS-250
  • FPLC, GE Life Sciences AKTA purifier
  • Isothermal titration calorimeters, Microcal ITC200 and VP-ITC
  • Stopped-flow system, BioLogic SFM-20

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