NMR (La Jolla)

Overview

The NMR facility at Sanford-Burnham is a centralized shared resource that offers the capability for NMR studies on proteins, peptides, small molecules, and carbohydrates in solution or in solid state.

The Facility provides instrumentation and expertise for NMR data collection. It also provides consultation with investigators on the feasibility of NMR for structural studies of protein candidates, as well as the optimal method to obtain solution structures and binding information by multi-dimensional NMR techniques. It can also train users in basic spectrometer operations, trouble-shoot for instrumental and operational problems, and set up NMR experiments for users as requested.
Tech - Shared Resources: ServicesQuickJump

Services

  • Maintaining the spectrometers for self-usage by experienced user, trouble shooting for problems
  • Collecting and processing NMR data according to users specifications
  • Training users for basic NMR experiments

Equipment and Resources

  • NMR600: 600 MHz Bruker Avance Spectrometer with 5 mm TCI cryoprobe, for high resolution structural studies of macromolecules
  • NMR500: 500 MHz Bruker Avance Spectrometer with automatic sampler changer, several optional probes (5 mm TXI, z-gradient, 5 mm BBO z-gradient, 5 mm 19F-1H), for structural studies and NMR-based drug discovery
  • Solid500: 500 MHz Bruker Avance solid-state NMR spectrometer, for high resolution structural studies of membranes proteins
  • 400MHz JEOL JNM-ECS spectrometer, with a 5 mm proton/multi-frequency auto-tune and match-ready probe, and an auto sample changer
  • nmrlinux: Linux computer for data processing and back-up

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