Histopathology (La Jolla)

Overview

The Histopathology Core Facility provides standard and customized research specific histology services including tissue processing, fixed and frozen sectioning. The Facility also offers routine and special histological stains as well as immunohistochemistry and immunofluorescence. The Facility maintains and provides training for several pieces of common use equipment, such as, paraffin microtome, cryostat, and a cytospin. Training is also provided for basic histological techniques such as, perfusion and tissue collection. Finished slides are returned to the researcher for evaluation, or they may be digitized using our latest addition, the Aperio Scanscope. The Aperio allows not only digitizing of slides for archiving, it also offers powerful image analysis tools as well.
Tech - Shared Resources: ServicesQuickJump-PricesOnly

Services

Conventional histology

    • Routine and custom sectioning
    • Paraffin and frozen samples
    • Standard and special stain (H&E, PAS, silver impregnation)

Immunohistochemistry

    • Development of custom protocols (overlay assays, competition assays)
    • Titration and staining of commercial and custom antibodies
    • Double labeling of antibodies
    • Immunofluorescence

Training

Equipment and Resources

  • Leica CM 3050 cryostat
  • Leica RM 2125 paraffin microtome
  • Dako autostainer plus
  • Shandon Cytospin 3
  • Sakura Tissue Tek vacuum infiltration tissue processor
  • Leica EG 1160 paraffin embedding station
  • MMI Cell Cut Laser Microdissection system
  • Aperio Scanscope XT system

Price List
Additional Sections Frozeneach slide$5.50$6.88$7.43$14.47
Additional Sections/ paraffinper slide$5.50$6.88$7.43$14.47
Antibody Titration and Testingper antibody$150.00$187.50$202.50$394.50
Aperio 20X scanper slide$10.00$12.50$13.50$26.30
Aperio 40X scanper slide$20.00$25.00$27.00$52.60
Aperio Access Fee (1 time charge)per lab$300.00$375.00$405.00$789.00
Aperio Fluorescence Scanper slide$20.00$25.00$27.00$52.60
Aperio Macro Developmenteach$60.00$75.00$81.00$157.80
Common Cryostatper hour$20.00$25.00$27.00$52.60
Common Microtomeper hour$20.00$25.00$27.00$52.60
Cytospinper run$10.00$12.50$13.50$26.30
Deparaffinizing/Antigen RetrievalPER run$5.00$6.25$6.75$13.15
Embedding Center useper hour$15.00$18.75$20.25$39.45
Embedding Cryoeach$6.00$7.50$8.10$15.78
Embedding Only/ Paraffineach$6.50$8.13$8.78$14.47
H&E Stainingper slide$6.00$7.50$8.10$15.78
IHC benchtimeper hour$15.00$18.75$20.25$39.45
Immuno Double labelingper slide$85.00$106.25$114.75$223.55
Immuno Staining (IHC or IF)per slide$35.00$43.75$47.25$92.05
Large Combo/Embed/H&E/10unstainedeach$27.50$34.38$37.13$72.33
Large Slide Boxeach$18.00$22.50$24.30$47.34
Laser Capture Microscopeper hour$20.00$25.00$27.00$52.60
LCM sample prepeach$15.00$18.75$20.25$39.45
Materials (Dollar for Dollar)each$1.00$1.25$1.35$2.63
Neurohistologyper hour$35.00$43.75$47.25$92.05
Perfusioneach$30.00$37.50$40.50$78.90
Rnase Free Sectioningeach slide$6.00$7.50$8.10$15.78
Slide Foldereach$18.00$22.50$24.30$47.34
Small Combo/Embed/H&E/5ustainedeach$17.50$21.88$23.63$46.03
Small slide Boxeach$10.00$12.50$13.50$26.30
Special Stainingper slide$12.50$15.63$16.88$32.88
Technical Assistanceper hour$60.00$75.00$81.00$157.80
Tumor Bank sample request from inventoryEach
Tumor Bank sample special requesteach$60.00$75.00$81.00$157.80
TUNEL Assayper slide$40.00$50.00$54.00$105.20
Vibratomeper hour$20.00$25.00$27.00$52.60
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