Flow Cytometry (La Jolla)

Services

High speed cell sorting

• Performed by facility personnel in two locations
• Single-cell (clone) sorting into 96 or 384-well plates
• Simultaneous sorting of up to 4 populations
• BSL2 biosafety enclosures
• Expertise in calcium flux, cell cycle, side population, and sorting of IPSC and ESC

Analytical flow cytometry

• Do-it-yourself 24hr/day, or assisted by appointment
• Hardware and software training
• Consultation
• Experiment design
• Data analysis and interpretation
• Publication quality figures
• Assistance with methods sections
• Pre-publication manuscript review

Equipment and Resources

• BD FACSAria 12-color high-speed cell sorter
  407 nm, 488 nm, and 633 nm laser lines
• BD FACSDiVa 7-color high-speed cell sorter
  Argon (488 nm or 514 nm) Krypton (multi-line UV, 407 nm or 568 nm) and HeNe (633 nm) lasers
• BD LSRFortessa 14-color FACS analyzer
  HTS plate loader for 96 or 384-well plates
  four solid state lasers (405 nm, 488 nm, 561 nm, 640 nm)
• BD FACSCanto 6-color FACS analyzer
  488 nm and 633 nm lasers
  Typical fluor combinations: FITC, PE, PerCP, PE-Cy7, APC, APC-Cy7 or equivalent
• BD FACSCalibur 4-color analyzer
  488 nm and 635 nm lasers
  Typical fluor combinations: FITC, PE, PerCP, APC or equivalent
• BD FACSort 3-color FACS analyzer
  488 nm excitation
  Typical fluor combinations: FITC, PE, PerCP or equivalent
• LifeTechnologies Countess Automated Cell Counter
• BioRad TC10 automated cell counter
• Computer workstations (Macintosh OSX) with large monitors and data analysis software packages including
  FlowJo
  ModFit LT
  CellQuest
  FACSDiVa

Contact

Facility Director
Yoav Altman

• Email: yoav@sanfordburnham.org
• Phone: 858-646-3100 x3569

Scientific Director
Mark Mercola, Ph.D.

• Email: mmercola@sanfordburnham.org

Our flow cytometry equipment and expertise is available to the entire San Diego scientific community. We emphasize customer service and will work with you before, during and after your experiment to get the best results possible. Your satisfaction is our highest priority.

To schedule sorting or analysis appointments or request hardware training visit our iLab webpage. New users will need to sign up for an iLab account.

Emails sent to facs@sanfordburnham.org reach all core lab personnel.

Price List

Links

Sanford-Burnham Flow Cytometry

• iLab
• Facebook

References and Journals

• Current Protocols in Cytometry
• Cytometry Part A, the journal of the International Society of Analytical Cytometry

Protocols

• Sort Buffer Recipe - TSRI
• Antibody Titration - UCSD Cancer Center
• Antibody Titration - University of Chicago