Cellular Imaging (La Jolla)

Services

• Single and multiphoton confocal microscopy
• Live cell imaging
• Wide field microscopy
  • bright field and dark field microscopy
  • phase and Nomarski differential interference contrast
  • polarization and epi-fluorescent microscopy
• Electron microscopy
• Image analysis:
  • morphometry
  • 3D and 4D rendering
  • deconvolution
• Training and consultation

Equipment and Resources

Confocal Microscopy

• LSM 710 NLO Zeiss Multiphoton Laser Point Scanning Confocal Microscope
  Five Single Photon Lasers (458, 488, 514, 561 & 633 nm);
  Multi-photon Mai-Tai Laser HB (690-1024nm) dedicated for excitation of
  multiple fluorophores, including UV excited dyes, such as DAPI.
  CO2 & Thermo Controlled Time lapse system
• Fluoview 1000 Olympus Laser Point Scanning Confocal Microscope
  Single Photon Lasers: Diode (405nm); Ar-ion laser (457, 488, 514 nm);
  DPSS laser (561 nm); He-Ne laser (633 nm);
  Dual SIM Scanner for simultaneous stimulation & registration;
  Spectral Deconvolution; FRAP; FRET; Photoactivation applications;
  CO2 & Thermo Controlled Time lapse system
• Yokogawa Spinning Disk Laser Confocal Microscope
  XY and Piezo-Z object stage (ASI 2000-Piezo);
  Kr-Ar laser (488, 568 and 647 nm)(Melles Griot);
  Cooled Monochrome CCD QuantE Camera (Photometrics).
  MetaMorph (Mol. Devices) software.
  CO2 & Thermo Controlled Time lapse system

Wide Field Microscopy

• Inverted IX81 Olympus Wide Field and Fluorescence Microscope (FRET)
  FRET & Ca2+ Imaging, DIC. XY-linear encoded objective stage ASI 2000(ASI Inc.); Z-IX81 step-motor.
  Cooled Monochrome CCD Cascade 512B FT Camera. (Photometrics Inc.).
  DG4 UV source galvanometer, 350W Xenon lamp, 4 chanels & Emis. Fiter Wheel (Sutter).
  MetaMorph software, v. 7.7.7 (Mol. Devices).
  CO2 & Thermo Controlled Time lapse system
• Inverted IX81 Olympus Wide Field and Fluorescence Microscope (TIRF)
  XYand Piezo-Z object stage ASI 2000 (ASI Inc.);
  Cooled, Monochrome CCD QuantEM Camera (Photometrics Inc.)
  CO2 & Thermo Controlled Time lapse system
• Inverted IX81 Olympus Wide Field and Fluorescence Microscope
  XY-Martzhauser Tango objective stage (Martzhauser Inc.); Z-IX81 step-motor.
  Cooled Monochrome ORCA ER (Hi Res) camera (Hamamtsu).
  X-cite 120 Metal Halide UV source (EXFO).
  Excit.& Emis.Filter Wheels (Sutter).
  ImageJ Microimager.
  CO2 & Thermo Controlled Time lapse system
• Inverted TE300 Nikon Wide Field, Fluorescence Microscope #1
  Multiple Fluorescence; Phase and Nomarski DIC contrast;
  Cooled, Color CCD SPOT RT Camera (Diag.Instruments).
• Inverted TE300 Nikon Wide Field, Fluorescence Microscope #2
  Multiple Fluorescence; Nomarski (DIC) contrast;
  XYZ object stage ASI 2000 (ASI Inc.);
  Cooled Color CCD SPOT RT Camera(Diag.Instruments).
  Thermo Controlled Time lapse system
• Upright BX50 Olympus Wide Field, Fluorescence Microscope
  Transmitted light and Triple Fluorescent imaging.
  Cooled, Color CCD SPOT RT Camera (Diag.Instruments).

• Electron Microscope Hitachi H-600A
  Cooled 11.2Megapixel CCD camera SIA-L9C.
  Maxim DL acquisition software.
• Software Packages for Image Processing
  ImagePro Plus (MediaCybernetics)
  Volocity 4D Rendering (Improvision)
  Spot RT Acquisition / Processing Software (Diagnostic Instruments Inc.)
  ImageJ and IrfanView (free sources imaging packages)

Price List