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Cell-based Disease Modeling and Screening (La Jolla)
Overview
The Cell-based Disease Modeling and Screening Core supports development of cell based disease models for screening and testing small molecules and drugs, and specializes in culture, scaling, and directed differentiation of human embryonic and induced pluripotent stem cells. We provide resources and expertise to researchers at the Institute and to our external partners to facilitate development of stem cell based assays and patient-specific disease models in screenable formats. We have recently completed an ultra high throughput 365,000 compound screen of human induced pluripotent stem cells (hiPSC), and developed and screened a patient-specific cell based disease model for muscular dystrophy. |
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Equipment and Resources
Our BSL-2-certified laboratory for stem cell culture is equipped with two biosafety cabinets, an EnvirCo laminar flow open bench hood for the manual manipulation of cultures, a Leica MZ6 Stereomicroscope and a CKX41SF2 Olympus inverted microscope, both with digital camera set ups, platform rotators, a Biospherix hypoxia chamber, and four CO2 incubators. In addition to the stem cell laboratory, the CPCCG’s sterile environment facility includes a second BSL-2 standard tissue culture room. |
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Novel surface markers for the isolation and transplantation of pancreatic cells derived from human embryonic stem cells.
Kelly O.G., M.Y. Chan, L.A. Martinson, K. Kadoya, T. Ostertag, K. Ross, M. Richardson, M.K. Carpenter, K.A. D’Amour, E. Kroon, M. Moorman, E.E. Baetge, and A.G. Bang.
Nat Biotechnol. 2011 Jul 31;29(8):750-6. doi: 10.1038/nbt.1931.
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Characteristics and characterization of human pluripotential stem cells.
Bang A.G and M.K. Carpenter
In Essentials of Stem Cell Biology, Ch. 38, 2nd Edition, ed. Robert Lanza, Academic Press. 2009.
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Pancreatic endoderm derived from human embryonic stem cells generates glucose responsive insulin-secreting cells in vivo.
Kroon E., L. A. Martinson, K. Kadoya, A.G.Bang, O.G. Kelly, S. Eliazer, H. Young, M. Richardson, N.G. Smart, J. Cunningham, A.D. Agulnick, D'Amour KA, M.K. Carpenter & E.E. Baetge
Nat Biotechnol. 2008 Apr;26(4):443-52. Epub 2008 Feb 20.
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Deconstructing pluripotency. Commentary.
Bang A.G and M.K. Carpenter
Science. 2008 Apr 4;320(5872):58-9.
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Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells.
D'Amour K.A,. A.G. Bang, S. Eliazer, O.G. Kelly, A.D. Agulnick, N.G. Smart, M.A. Moorman, E. Kroon, M.K. Carpenter & E.E. Baetge
Nat Biotechnol. 2006 Nov;24(11):1392-401. Epub 2006 Oct 19.
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Tech - Shared Resources: Prebys Center Links Related
Links to Related Sanford-Burnham Core Services
The Conrad Prebys Center of Chemical Genomics provides a comprehensive suite of capabilities
for preclinical small molecule drug discovery by providing specialized expertise within our eight core facilities:
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