Pouring Gradient Gels:
multi-caster (large gel) for Amersham Pharmacia SE 600 system.
large gels uses about 100 ml of gel volume.
gels in caster.
to make sure all tubing etc works.
50ml solution add 165ml of 10% APS and 16.5ml
pump speed at 48 (about 10 mls per minute)
tubing when air bubbles begin forming in tubing towards end. Add water to mixer to prevent
polymerization of acrylamide in the mixer chambers.