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As a resource for the Burnham Institute and being a member of the La Jolla Interdisciplinary Neuroscience Consortium, the SCCF offers reagents, cell-lines, feeders and services that one will require for the maintenance, differentiation and manipulation of their stem cells. Below you will find the list of supplies and cell-lines that we offer at the indicated price.

Manufacturer	Product	Catalog #	Aliquot Size	Price Each
Gibco	DMEM-F12 + Glutamax	10565-018	500 ml	$13.50
Gibco	K.O DMEM	10829-018	500 ml	$25.00
Cellgro	DPBS	21-031-CV	500 ml	$5.00
Cellgro	H2O	25-055-CV	500 ml	$5.00
Gibco	KOSR	10828-028	50 ml	$30.00
Hyclone	FBS	SV30014.03	50 ml	$23.75
Sigma	bfgf (25 ng/ul)	F0291	100 ul	$20.00
Invitrogen	GFR Geltrex (1mg/ml)	12760-021	1 ml (12 wells)	$3.50
BD	GFR Matrigel (1mg/ml)	356231	1 ml (12 wells)	$8.50
Stem Cell Tech.	mTeSRI + Antibi/mycotic	5850	25 ml	$20.00
Gibco	Collagenase IV (1 mg/ml)	17104-019	5 ml	$2.50
Gibco	NEAA 100x	11140-050	5 ml	$1.50
Gibco	Beta-mercapto (55 nM)	21985-023	5 ml	$4.50
Gibco	Glutamax 100x	35050-061	5 ml	$1.50
Gibco	Trypsin .25/edta	25200-056	5 ml	$1.50
Gibco	Pen/Strep 100x	15070-063	5 ml	$1.50
Gibco	Antibi./Antimy. 100x	15240-062	5 ml	$1.50
Gibco	Sodium Pyruvate 100x	11360-070	5 ml	$1.50
Stem Cell Tech.	0.1% Gelatin	07903	10 ml	$1.00
ATCC	DMSO--Cell Culture Grade	4-X	5 ml	$10.25
Sigma	DMSO--HybriMax	D2650	5 ml	$10.25
CalBio Chem	Rock Inhibitor (10 mM)	Y-27632	25 ul	$17.50
Stem Cell Tech.	Dispase 5 mg/ml	07913	5 ml	$10.00
Millipore	Accutase	SCR005	5 ml	$3.50
Corning	24 well low attachment	3473	1 plate	$12.50
Corning	6 well low attachment	3471	1 plate	$9.50
Nunc	V96 well plate	249935	1 plate	$3.50
	Reagents for Roche Lightcycler 480
Roche	96 well plate		1 ea	$4.50
Roche	384 well plate		1 ea	$5.00
Roche	1 ml Sybr Green Master Mix & 1 ml PCR Grade H2O		1 ml	$35.00
	1 ml Sybr Green Master Mix & 1 ml PCR Grade H2O		1 ml	$35.00

	Stem Cells & Feeders
wt c57/bl6 mEFs	3 x 10^6 irradiated		1 Vial	$25.00
wt c57/bl6 mEFs	1.5 x 10^6 irradiated		1 Vial	$12.50
mEF Cond. Media	Cultured 48 hrs - (0.2 uM)		45 ml	$31.50

HS-27	3 x 10^6 irradiated		1 Vial	$25.00
HS-27	1.5 x 10^6 irradiated		1 Vial	$12.50
HS-27 Cond. Media	Cultured 48 hrs - (0.2 uM)		45 ml	$31.50

NIH Approved Lines	1 Vial (~2 x 10^6)		1 Vial	$100.00
NIH Approved LInes	1 split (Growing)		1 Split	$100.00



	Non-NIH Stem Cell Lines
	Please Inquire			Inquire

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**Transfer of hESC lines between institutions is dependant upon proper MTA's being in place**