Senior Research Associate/Lab Manager
Plasma Cell Formation, Isotype Switching, and IL-4 Responses
My work focuses on elucidating the role of CD19 in plasma cell
formation utilizing in vitro culture systems of purified splenic B cells from
CD19-/- mice. Through detailed evaluation including flow cytometric analysis,
ELISA, ELISPOT, qRT PCR, viral transduction systems and biochemical techniques a
role for CD19 in coordinating phenotypic changes associated with plasma cell
differentiation formation was determined. Related work on Blimp-1,
the “master regulator” of plasma cell formation suggests a role for Blimp the
development or maintenance of B-1 cells.
Additional projects and collaborations:
Role of PI3K and CD19 in T cell dependent B cell immune responses including
plasma cell differentiation and isotype class switch recombination in the
germinal center; including regulation of PI3K by the lipid phosphatases PTEN and
SHIP as well as control of the downstream transcriptional regulators (such as
BLIMP-1) involved in these processes.
In collaboration with Sidne
Cyclin D2 and Cyclin D3 in T cell dependent B cell immune responses.
In collaboration with
Suresh Chintalapati, PhD
Ephrin receptor signaling and the role of Ephrin family receptors, ligands and
substrates (such as SHEP-1) on B cell homing and adhesion related to proper B
cell development and the immune response.
In collaboration with Cecille
Browne and Elena Pasquale, PhD
Other collaborations at The Sanford-Burnham Institute:
Specific in vivo targeting of small walled carbon nanotubes and other nano-materials
to specific cells types.
In collaboration with Massimo Bottini, PhD and Thomas Mustelin,
Determination of the function of the ubiquitin ligase Rnf5a in
lymphocyte activation, the normal immune response, and autoimmunity.
In collaboration with
Agnes Delaunay-Moisan, PhD and Ze’ev
2004, MS, Biology, University of California, San Diego
Molecular Biology Major, University of California, San Diego
Certificate, Biotech Project Management, University of Washington/University of
California, San Diego.
Cato, M.H.,* Yau, I.W. * and R.C. Rickert. 2011.
Magnetic-based purification of untouched mouse germinal center B cells for ex-
vivo manipulation and biochemical analysis. Nature Protocols.
9;6(7):953-60. doi: 10.1038/nprot.2011.344.
Cato, M.H., S.K. Chintalapati, I.W. Yau, S.A. Omori
and R.C. Rickert. 2011. Cyclin D3 is selectively required for proliferative
expansion of germinal center B cells. Mol. Cell. Bio. (1):127-37 .
Baracho GV, Miletic AV, Omori SA, Cato MH, Rickert
RC. 2011. Emergence of the PI3-kinase pathway as a central modulator of normal
and aberrant B cell differentiation. Curr Opin Immunol. (2):178-83.
Browne, C.D., M.M. Hoefer, S.K. Chintalapati, M.H.
Cato, Y. Wallez, E.B. Pasquale and R.C. Rickert. 2010. SHEP1 partners with CasL
to promote marginal zone B cell maturation. Proc. Natl. Acad. Sci. USA.
Li S, Ezhevsky S, Dewing A, Cato MH, Scortegagna M,
Bhoumik A, Breitwieser W, Braddock D, Eroshkin A, Qi J, Chen M, Kim J, Jones S,
Jones N, Rickert RC, Ronai ZA. 2010. Radiation Sensitivity and Tumor
Susceptibility in ATM phospho-mutant ATF2 mice. Genes and Cancer.
Browne, C.D., C.J. Del Nagro, M.H. Cato, S.H. Dengler and
R.C. Rickert. Suppression of PI(3,4,5)P3 production is a key
determinant of B cell anergy.
F. D'Annibale, D.M. Mills, F. Cerignoli, M.I. Dawson, E. Bergamaschi, N.
Bottini, A. Magrini, A. Bergamaschi A, N. Rosato, R.C. Rickert, T. Mustelin, and
M. Bottini. 2008.
Cell-type specific and cytoplasmic targeting of PEGylated carbon nanotube-based
nanoassemblies. J. Nanosci. Nanotechnol. 8(5):2259-69.
Omori, S.A., M.H.
Cato, A. Anzelon-Mills, K.D. Puri, M. Shapiro-Shelef, K. Calame, and R.C.
Rickert. (2006). Regulation of class switch recombination and plasma cell
differentiation by the PI3K/Akt/Foxo signaling axis. Immunity,
25:545-557. (Highlighted in Science’s STKE, 2006, tw365).