Dr. Jiang focuses on insulin signal networks related to glucose metabolism.
Dr. Jiang received his M.S. in pharmacology from Peking Union Medical College and a Ph.D. in biochemistry from the Univeristy of London.
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Akt substrate TBC1D1 regulates GLUT1 expression through the mTOR pathway in 3T3-L1 adipocytes.
Zhou QL, Jiang ZY, Holik J, Chawla A, Hagan GN, Leszyk J, Czech MP
Biochem J. 2008 May 1;411(3):647-55
Identification of WNK1 as a substrate of Akt/protein kinase B and a negative regulator of insulin-stimulated mitogenesis in 3T3-L1 cells.
Jiang ZY, Zhou QL, Holik J, Patel S, Leszyk J, Coleman K, Chouinard M, Czech MP
J Biol Chem. 2005 Jun 3;280(22):21622-8
Insulin signaling through Akt/protein kinase B analyzed by small interfering RNA-mediated gene silencing.
Jiang ZY, Zhou QL, Coleman KA, Chouinard M, Boese Q, Czech MP
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7569-74
Characterization of multiple signaling pathways of insulin in the regulation of vascular endothelial growth factor expression in vascular cells and angiogenesis.
Jiang ZY, He Z, King BL, Kuroki T, Opland DM, Suzuma K, Suzuma I, Ueki K, Kulkarni RN, Kahn CR, King GL
J Biol Chem. 2003 Aug 22;278(34):31964-71
Glucose transporter recycling in response to insulin is facilitated by myosin Myo1c.
Bose A, Guilherme A, Robida SI, Nicoloro SM, Zhou QL, Jiang ZY, Pomerleau DP, Czech MP
Nature. 2002 Dec 19-26;420(6917):821-4
A phosphatidylinositol 3-kinase-independent insulin signaling pathway to N-WASP/Arp2/3/F-actin required for GLUT4 glucose transporter recycling.
Jiang ZY, Chawla A, Bose A, Way M, Czech MP
J Biol Chem. 2002 Jan 4;277(1):509-15
Characterization of selective resistance to insulin signaling in the vasculature of obese Zucker (fa/fa) rats.
Jiang ZY, Lin YW, Clemont A, Feener EP, Hein KD, Igarashi M, Yamauchi T, White MF, King GL
J Clin Invest. 1999 Aug;104(4):447-57
Zhen Jiang's Research Focus
Metabolic Syndrome, Obesity, Diabetes - General
Our research focuses on insulin signal networks related to glucose metabolism. Currently, we are using TIRF microscopy-based live cell imaging, protein-protein interactions and a knockout mouse model to study the role of CDP138, a novel phosphoprotein containing C2 domain, in GLUT4 translocation, glucose metabolism and neuronal functions. We are also interested in applying a serum proteomic approach to identify differentially expressed serum proteins in obese insulin-resistant animal models and their role in obesity-related complications.
Zhen Jiang's Research Report
Our research focuses on insulin signal networks related to metabolism. Insulin has pleiotropic actions in
the human body including the regulation of glucose metabolism, fat storage, protein synthesis and gene expression. Many of these metabolic functions of insulin are mediated through the PI 3-kinase pathway. Studies reported by us and others have suggested that insulin signaling in the PI 3-kinase pathway is selectively impaired under insulin resistant states, such as
in obesity animal models and type II diabetes mellitus. Using an siRNA-mediated gene silencing approach we observed that a PI 3-kinase downstream protein kinase Akt, particularly Akt2, is absolutely required for insulin-stimulated glucose uptake and the translocation of glucose transporter GLUT4 from its intracellular storage to the plasma membrane in adipocytes. Together, our studies and others support the notion that the PI 3-kinase – Akt2 pathway plays a critical role in the regulation of glucose metabolism both at normal and diabetic states. Recently, our efforts
have been directed at identifying the key cellular components that link Akt signaling to glucose transport, adipokine secretion and adipogenesis. In collaboration with Dr. Marcus Krueger in Dr. Matthias Mann's laboratory at the Planck Institute of Biochemistry
in Munich, Germany, we applied a SILAC-based quantitative proteomics approach and successfully identified more than 100 different phosphoproteins and their interacting proteins from insulin-stimulated adipocytes. Currently, our lab uses multiple approaches including RNAi-based gene silencing, protein-protein interaction, proteomics, live cell imaging and transgenic mouse models to dissect Akt signal networks involved in glucose metabolism and adipogenesis. In addition, our laboratory is also applying
a proteomic approach to identify biomarkers of obesity and diabetes.
About Zhen Jiang
Zhen Y. Jiang, M.S., M.D., Ph.D., was trained as a Medical Doctor before earning his M.S. in pharmacology from Peking Union Medical College, China, and a Ph.D. in biochemistry from University College, the University of London, UK. He did his postdoctoral research training at the Joslin Diabetes Center, Harvard Medical School in Boston before working as an Instructor and then a Research Assistant Professor in the Program in Molecular Medicine at the University of Massachusetts Medical School. Dr. Jiang was recruited to the Diabetes and Obesity Research Center at Sanford-Burnham Medical Research Institute as an Assistant Professor in 2008.